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KMID : 1145120160140010078
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Asian Journal of Beauty and Cosmetology
2016 Volume.14 No. 1 p.78 ~ p.88
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Evaluation of Water Extract Prepared from Chrysanthemum indicum Linne as Nutri-cosmetic and Cosmetic Material In Vitro Model
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Kim Ryeong-Hyeon
Song Ji-Hye
Shon Myung-Soo
Chun Kae-Shik
Choi Sun-Uk
Kim Gyo-Nam
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Abstract
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Purpose: Chrysanthemum indicum Linne (CI) has been used as a traditional medicine. Although several biological effects of CI have been reported, the precise role and function of CI as nutri-cosmetic and cosmetic materials remain unclear. Therefore, we prepared water extract of Chrysanthemum indicum Linne (CIW). And nutri-cosmetic potential was discussed through evaluation of anti-oxidant, anti-adipogenic in 3T3-L1 cells and regulatory function of skin fibril-related genes in human skin fibroblast (HSF).
Methods: The anti-oxidant activity of CIW was evaluated through metal chelating activity and reduction potential. The cytotoxicity of CIW in 3T3-L1 and HSF was evaluated by MTT assay. The anti-adipogenic effect of CIW was examined by Oil Red O (ORO) staining and microscopy observation in 3T3-L1 cells. The mRNA expression levels of skin fibril-related genes in HSF were analyzed by reverse transcription-polymer chain reaction (RT-PCR).
Results: The anti-oxidant activity of CIW was increased in a dose dependent manner. The CIW treatment up to 100 μg/mL for 24 h did not affect to the viability of 3T3-L1 cells and HSF. Thus, up to 100 μg/mL of CIW was chosen in cell-based assay. The treatment of 10, 50, and 100 μg/mL CIW significantly inhibited 3T3-L1 adipogenesis by 5.17%, 72.80%, and 104.09%. In addition, upregulated mRNA expression levels of skin fibril-related genes such as fibronectin and type Ⅰ collagen α2 (COL1A2) were observed in CIW-treated HSF.
Conclusion: These results suggest that CIW has the potential as nutri-cosmetic and cosmetic ingredient which possess anti-oxidant and anti-adipogenic effects.
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KEYWORD
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Chrysanthemum indicum Linne, Skin, Anti-oxidant, Adipogenesis, Nutri-cosmetics
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